Product Info Summary
SKU: | PA1925 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-MEKK3/MAP3K3 Antibody
SKU/Catalog Number
PA1925
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MEKK3/MAP3K3 Antibody catalog # PA1925. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEKK3/MAP3K3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1925)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse MEKK3, different from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1925 is reactive to MAP3K3 in Mouse, Rat
Applications
PA1925 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
71 kDa
Calculated molecular weight
70.898kDa
Background of MEKK3
MAP3K3 (Mitogen-activated protein kinase kinase kinase 3), also known as MAP/ERK KINASE KINASE 3, MEKK3 or MAPKKK3, is an enzyme that in humans is encoded by the MAP3K3 gene. MAP3K3 is contiguously distal to LYK5 on chromosome 17 (Puffenberger et al., 2007). By yeast 2-hybrid analysis of a mouse T-cell cDNA library, Uhlik et al. (2003) showed that a C-terminal fragment of mouse Osm (CCM2) interacted with Mekk3, which activates p38 in response to sorbitol-induced hyperosmotic conditions. Mekk3 and Osm colocalized in the cytoplasmic compartment of cotransfected cells, and the Mekk3-Osm complex was recruited to Rac1 and cytoskeletal actin-containing membrane ruffles in response to sorbitol treatment. Protein interaction assays showed that Osm interacted directly with the Mekk3 substrate Mkk3 (MAP2K3), with actin, and with both GDP- and GTP-loaded Rac1. Uhlik et al. (2003) concluded that the RAC1-OSM-MEKK3-MKK3 complex is required for regulation of p38 activity in response to osmotic shock.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Validation Images & Assay Conditions
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Anti-MEKK3 antibody, PA1925, IHC(P)
IHC(P): Rat Brain Tissue
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Anti-MEKK3 antibody, PA1925, IHC(P)
IHC(P): Rat Brain Tissue
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Anti-MEKK3 antibody, PA1925, Western blotting
All lanes: Anti MAP3K3 (PA1925) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 71KD
Observed bind size: 71KD
Protein Target Info & Infographic
Gene/Protein Information For MAP3K3 (Source: Uniprot.org, NCBI)
Gene Name
MAP3K3
Full Name
Mitogen-activated protein kinase kinase kinase 3
Weight
70.898kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11.25; MAP/ERK kinase kinase 3; MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MAPKKK3MEKK 3; MEK kinase 3; MEKK3; mitogen-activated protein kinase kinase kinase 3 MAP3K3 MAPKKK3, MEKK3 mitogen-activated protein kinase kinase kinase 3 mitogen-activated protein kinase kinase kinase 3|MAP/ERK kinase kinase 3|MAPK/ERK kinase kinase 3|MEK kinase 3|MEKK 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP3K3, check out the MAP3K3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP3K3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEKK3/MAP3K3 Antibody (PA1925)
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No publications found for PA1925
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-MEKK3/MAP3K3 Antibody
Question
We are currently using anti-MEKK3/MAP3K3 antibody PA1925 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
The anti-MEKK3/MAP3K3 antibody (PA1925) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-17